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PC cells were harvested after trypsinization, followed by centrifugation and then fixed at 4 C overnight in Karnovskys fixative (2% paraformaldehyde and 5% glutaraldehyde in 0.1 M cacodylate, pH 7.4)

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Of these, only 33 percent (16 percent of responding hospitals overall) reported reaching discharged patients most of the time. Further, among hospitals that furnish follow-up contacts, fewer than half reported covering any of the main aims of FCI, for example, 41 percent review the Safety Plan, 49 percent provide psychosocial support, and 38 percent facilitate outpatient care