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For first-strand cDNA synthesis, 500 ng of total RNA were used with an equimolar mixture of four oligo(dT) primers (CDSIII-NotIA/C/G/T, Supplementary Table 7) and RevertAid H Minus Reverse Transcriptase (Thermo Scientific)

In resting cells, ZDHHC7-mediated constitutive palmitoylation of NLRP3 lowers the threshold for phase separation, thereby enabling cellular responsiveness to diverse stimuli (including intracellular perturbations and NLRP3-binding molecules) to trigger NLRP3 activation

Antioxidant protection: GHK-Cu peptides neutralize harmful molecules that can age skin prematurely

doi: 10.1371/journal.pone.0085544

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